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| Registros recuperados: 23 | |
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| Dufourg, Cecile. |
| Les 2 taxons d'huîtres creuses C. angulata et C. gigas n'étant pas différenciables sur des critères morphologiques, écologiques ou physiologiques, l'étude de leur passé évolutif fait appel à l'utilisation de marqueurs génétiques. L'étude du polymprohisme des séquences mitochondriales codant pour la sous-unité I de la cytochrome oxydase par la technique de PCR-RFLP a été réalisée sur 197 individus appartenant à 5 populations d'origine géographique différente, de type Crassostrea angulata ou Crassostrea gigas. Elle a permis la construction d'un arbre représentant les relations phylogénétiques entre ces populations. Cet arbre a été comparé à celui établi à partir de l'analyse de 3 marqueurs microsatellites. Les résultats de ces 2 analyses sont similaires et... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea angulata; Crassostrea gigas; Génétique; Polymorphisme; ADN; SSCP; Biologie moléculaire. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00033/14415/11709.pdf |
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| Fajardo,Thor V.M.; Eiras,Marcelo; Schenato,Paula G.; Nickel,Osmar; Kuhn,Gilmar B.. |
| As principais espécies de vírus envolvidas na etiologia do enrolamento da folha da videira (Vitis spp.) são Grapevine leafroll-associated virus 1 e 3 (GLRaV-1 e -3). Neste estudo da variabilidade desses vírus, foram amplificados dois fragmentos de DNA (396 bp do GLRaV-1 e 602 bp do GLRaV-3) por RT-PCR, a partir de RNA total extraído de nervuras e pecíolos de videiras infetadas, utilizando-se dois pares de oligonucleotídeos. Os DNAs amplificados foram clonados e reamplificados, a partir dos clones recombinantes, e comparados quanto às diferenças conformacionais das fitas simples desnaturadas (SSCP). Foram observados dois padrões distintos de perfis eletroforéticos para cada vírus, tendo sido seqüenciado pelo menos um clone viral correspondente a cada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Videira; Vitis; GLRaV-1; GLRaV-3; SSCP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200013 |
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| Jabari,Linda; Gannoun,Hana; Khelifi,Eltaief; Cayol,Jean-Luc; Godon,Jean-Jacques; Hamdi,Moktar; Fardeau,Marie-Laure. |
| Abstract Wastewater from an anaerobic treatment plant at a slaughterhouse was analysed to determine the bacterial biodiversity present. Molecular analysis of the anaerobic sludge obtained from the treatment plant showed significant diversity, as 27 different phyla were identified. Firmicutes, Proteobacteria, Bacteroidetes, Thermotogae, Euryarchaeota (methanogens), and msbl6 (candidate division) were the dominant phyla of the anaerobic treatment plant and represented 21.7%, 18.5%, 11.5%, 9.4%, 8.9%, and 8.8% of the total bacteria identified, respectively. The dominant bacteria isolated were Clostridium, Bacteroides, Desulfobulbus, Desulfomicrobium, Desulfovibrio and Desulfotomaculum. Our results revealed the presence of new species, genera and families of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial ecology; Biodiversity; Bacterial culture; SSCP; PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100073 |
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| Hernández Rodríguez, Martha. |
| El maíz (Zea mays L.) de calidad proteínica (QPM) es nutricionalmente superior en comparación con el maíz normal, porque produce semillas con casi el doble de los aminoácidos (aa) lisina (Lys) y triptófano (Trp), esenciales para la síntesis endógena de la vitamina B niacina. Actualmente, la conversión de una línea de maíz normal a una QPM, se acelera mediante la selección asistida por marcadores moleculares (MAS) y métodos de detección química. En este estudio se reporta el desarrollo y validación de marcadores basados en polimorfismos de un solo nucleótido (SNPs) para los loci opaque-2 (o2) y Ask-2, involucrados en la genética de los QPM, así como la interacción de ambos loci para el contenido de 11 aa en el endospermo de dos poblaciones F2 de maíz,... |
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Palavras-chave: Zea mays; Opaque-2; Ask-2; MAS; QPM; SNP; SSCP; Genética; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2319 |
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| Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.. |
| We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008 |
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| Mezzadra,Carlos Alberto; Melucci,Lilia Magdalena; Corva,Pablo Marcelo; López Valiente,Sebastián; Rípoli,María Verónica; Lirón,Pedro; Giovambattista,Guillermo. |
| The influence of cytoplasmic inheritance on birth and weaning weight was evaluated in an experimental Hereford herd. Data on 1,720 records for birth and weaning weights from calves born between 1963 and 2002 were studied. Variance components were estimated using MTDFREML procedures and an animal model was fitted for each trait. Direct and maternal additive effects and permanent environment and maternal lineage effects were treated as random, while year and month of birth, age of dam and sex of the calf were treated as fixed. Identification of maternal lineages was based on pedigree information. The contribution to phenotypic variance of cytoplasmic lineages defined by pedigree information was negligible for both traits. Mitochondrial genotypes of cows... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beef cattle; Hereford; Birth weight; Weaning weight; Cytoplasmic inheritance; SSCP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300003 |
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| Rivas Valencia, Patricia. |
| La tristeza es causada por un complejo viral constituido por diversos haplotipos (hps) del Citrus tristeza virus (CTV). El objetivo fue realizar un análisis de la estructura poblacional en diferentes escenarios epidémicos: a) En Yucatán, México, en condiciones asintomáticas (As) y con presencia de T. citricida (Tc), vector asociado a aislamientos de tipo severo; y en Tamaulipas en condiciones As y sin Tc. b) En Brasil, en condiciones As y sintomáticas (s), en regiones con y sin muerte súbita de los cítricos (MSC), síndrome atribuible a una nueva variante severa del CTV. Se estudiaron las secuencias génicas p25 y p27 de la capa proteica viral. En México, con SSCP se demostró una estructura poblacional compleja en Tamaulipas, con 7 patrones... |
| Tipo: Tesis |
Palavras-chave: SSCP; Muerte súbita de los cítricos; Estructura poblacional; Doctorado; Fitopatología; Citrus sudden death; Population structure. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1368 |
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| Rivas Valencia, Patricia. |
| RESUMEN La tristeza es causada por un complejo viral constituido por diversos haplotipos (hps) del Citrus tristeza virus (CTV). El objetivo fue realizar un análisis de la estructura poblacional en diferentes escenarios epidémicos: a) En Yucatán, México, en condiciones asintomáticas (As) y con presencia de T. citricida (Tc), vector asociado a aislamientos de tipo severo; y en Tamaulipas en condiciones As y sin Tc. b) En Brasil, en condiciones As y sintomáticas (s), en regiones con y sin muerte súbita de los cítricos (MSC), síndrome atribuible a una nueva variante severa del CTV. Se estudiaron las secuencias génicas p25 y p27 de la capa proteica viral. En México, con SSCP se demostró una estructura poblacional compleja en Tamaulipas, con 7... |
| Tipo: Tesis |
Palavras-chave: SSCP; Muerte súbita de los cítricos; Estructura poblacional SSCP; Citrus sudden death; Population structure. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1061 |
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| Rivas-Valencia,Patricia; Loeza-Kuk,Emiliano; Mora-Aguilera,Gustavo; Febres,Vicente; Ochoa-Martínez,Daniel; Gutiérrez-Espinosa,Ma. Alejandra; Cintra de Jesus-Junior,Waldir; Correia-Malvas,Celia; Arno-Wulff,Nelson. |
| Se desconoce la etiología de la enfermedad muerte súbita de los cítricos (MSC) y su relación con una variante severa del virus tristeza de los cítricos (Citrus tristeza virus, CTV). Para estudiar una posible relación se estudiaron cinco poblaciones de 100 árboles cada una, en regiones con y sin presencia MSC, en huertas comerciales de los estados de São Paulo y Minas Gerais, Brasil. En las huertas con MSC se observó decoloración foliar, defoliación, crecimiento vegetativo reducido y eventualmente muerte del árbol. El objetivo de este estudio fue caracterizar la estructura poblacional de aislamientos de CTV presentes en ambas regiones con el fin de asociar etiológicamente al CTV con la MSC. Únicamente 96/500 muestras amplificaron para el gen de la capa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hibridación; Sondas específicas; SSCP. |
| Ano: 2008 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952008000100009 |
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| David, Elise; Boudry, Pierre; Degremont, Lionel; Tanguy, Amelie; Quere, N; Samain, Jean-francois; Moraga, D. |
| Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were studied in three sites on the French Atlantic coast (Baie-des-Veys, Auray and Ronce-les-Bains). The present paper examines the role of two candidate genes in the susceptibility of oysters to summer mortality, and the selective pressure exerted by such mortality on their polymorphism. Glutamine synthetase (amino-acid metabolism) and delta-9 desaturase (lipid metabolism) genes were studied in the successive generations, using polymerase chain reaction single-strand conformation polymorphism... |
| Tipo: Text |
Palavras-chave: SSCP; Mortality; Glutamine synthetase; Delta 9 desaturase; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3040.pdf |
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| Chen,Wen; Nie,Liu-wang; Zheng,Ping-ping. |
| The doublesex/male abnormal 3 (dsx/mab-3 or DM) domain gene family involved in sexual development encodes putative transcription factors including a DNA-binding homology motif, the DM domain. We used highly degenerate primers to clone and sequence seven distinct DM related transcription factor (Dmrt) genes from the Asian toad (Bufo gargarizans Cantor, 1842). A database search for the cloned sequences revealed the following percentage identity with the homologous Dmrt genes of the human: BgDmrt1 = 97%, BgDmrt2 = 97%, three isoforms of BgDmrt3 (BgDmrt3a = 93%, BgDmrt3b = 95%, BgDmrt3c = 100%) and two isoforms of BgDmrt5 (BgDmrt5 = 97%, BgDmrt5 = 91%). Based on DM domain amino acid sequence similarities we constructed a phylogenetic tree which grouped... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bufo gargarizans; Dmrt genes; DM domain; SSCP. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600026 |
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| Jie,Li; Zheng,Ping-Ping; Song,Jiao-Lian; Rui,Jin-Long; Nie,Liu-Wang. |
| The Sox family of genes shares a high sequence similarity with the HMG box region of the human Y chromosomal gene, SRY. We used highly degenerate primers to clone and sequence seven Eremias breuchleyi Sox genes (EbSox2, EbSox3, EbSox4, EbSox11, EbSox12, EbSox14 and EbSox21). A database search for the cloned sequences revealed the following percentage identity with the homologous human SOX genes: EbSox2 = 96%, EbSox3 = 88%, EbSox4 = 94%, EbSox11 = 99%, EbSox12 = 96%, EbSox14 = 98%, EbSox21 = 97%. Cluster analysis indicates that they seem to belong to group B and group C of Sox gene family, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eremias breuchleyi; PCR; Sequence analysis; Sox genes; SSCP. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300031 |
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| Wang,Ning; Wang,Jing J.; Jia,Rui; Xu,Jin W.; Nie,Liu W.. |
| Sox (SRY-related HMG-box) genes encode a family of transcriptional regulators, which are characterized by a conserved 79-amino acid domain known as HMG-box. They play essential roles in a diverse range of processes including sex determination and the development of the central nervous system (CNS), neural crest and endoderm. In this paper, the HMG domain of ten distinct Sox gene family members (os-Sox2, os-Sox3a, os-Sox3b, os-Sox4, os-Sox11a, os-Sox11b, os-Sox14a, os-Sox14b, os-Sox21a, os-Sox21b) were isolated from both male and female Odorrana schmackeri (Boettger, 1892) using PCR, and no sexual differences were found. Molecular phylogenetic analysis of the HMG domain suggested that these ten Sox genes are members of the SoxB and SoxC groups. In addition,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duplication-degeneration-complementation; SSCP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000100017 |
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| Otaviano,Antonio Roberto; Tonhati,Humberto; Sena,Janete Aparecida Desidério; Cerón Muñoz,Mario Fernando. |
| Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with changes in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffaloes; Polymorphism; PCR-RFLP; SSCP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200010 |
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| Sözen,Mehmet A.; Hecht,Jacqueline T.; Spritz,Richard A.. |
| Orofacial clefts (OFC; MIM 119530) are among the most common major birth defects. Here, we carried out mutation screening of the PVR and PVRL2 genes, which are both located at an OFC linkage region at 19q13 (OFC3) and are closely related to PVRL1, which has been associated with both syndromic and non-syndromic cleft lip and palate (nsCLP). We screened a total of 73 nsCLP patients and 105 non-cleft controls from the USA for variants in PVR and PVRL2, including all exons and encompassing all isoforms. We identified four variants in PVR and five in PVRL2. One non-synonymous PVR variant, A67T, was more frequent among nsCLP patients than among normal controls, but this difference did not achieve statistical significance. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: PVR; PVRL2; Cleft lip and palate; Mutation; SSCP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300007 |
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| Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio. |
| In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Neis genetic distance) suggest the existence of two genetically different groups, one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005 |
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| Mazzi,Carmen Maria; Ferro,Jesus Aparecido; Ferro,Maria Inês Tiraboschi; Savino,Vicente José Maria; Coelho,Antonio Augusto Domingos; Macari,Marcos. |
| The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler chickens; Hsp70 gene; Naked neck (Na) gene; Polymorphism; SSCP. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300010 |
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| Registros recuperados: 23 | |
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